We examined the pattern of cytokine secretion across the 24-hr day for women with widespread pain and tenderness having the diagnosis of fibromyalgia (FM) and matched healthy controls.  Subjects were given time to habituate to being in a clinical research laboratory environment and then were sampled for cytokines without their being disturbed for a 24-hr period including an 8-hr sleep period.  Cytokine levels were uniformly low but characterized by bursts of secretion.  Bursting occurred either in singlets or in doublets with a range from 88 to 131 mins between doublet bursts. There was an element of synchronization in these bursts with most occurring at the beginning of sampling.  FM patients showed a shift to increased IL-10 in the nighttime compared to controls.  The relation between this anti-inflammatory cytokine to the pro-inflammatory cytokines studied also differed between groups:  FM patients showed an increased ratio of IL-10 burst amplitude to that of pro-inflammatory cytokines IL-1β, IL-8 and TNF-α We interpret this to indicate a skew away from the normal balance favoring pro-inflammatory cytokines in controls toward one favoring an anti-inflammatory response in FM.  These changes toward anti-inflammatory predominance in FM may explain their common complaint of disturbed sleep because these cytokines are known to disrupt sleep.

Exp Bio Med (Maywood) (2009 Feb) 234(2):232-40